Lazio lose Joaquin Correa in the warm-up, while Atalanta start Ruslan Malinovskyi in this early showdown of potential Scudetto contenders.
It kicks off at the Stadio Olimpico at 19.45 UK time (18.45 GMT).
This Week 1 fixture was postponed from September 19-20 to give Atalanta extra time, as their season ended late due to the Champions League quarter-final against PSG.
Lazio began their season with a 2-0 victory away to Cagliari and coach Simone Inzaghi picked the same starting XI, largely due to the lack of alternatives.
However, a change was forced during the warm-up, as Correa pulled up with an injury and was replaced by Felipe Caicedo.
Luiz Felipe, Senad Lulic, Denis Vavro and Vedat Muriqi are still out of action, while Mohamed Fares and Wesley Hoedt haven’t completed their transfers yet.
Atalanta bring back Djimsiti after he missed the 4-2 win over Torino through suspension, sending Bosko Sutalo to the bench.
There are other changes, as Ruslan Malinovskyi starts with Papu Gomez supporting lone centre-forward Duvan Zapata.
Pierluigi Gollini, Matteo Pessina and Aleksei Miranchuk are injured.
Lazio: Strakosha; Patric, Acerbi, Radu; Lazzari, Milinkovic-Savic, Leiva, Luis Alberto, Marusic; Caicedo, Immobile
Atalanta: Sportiello; Toloi, Palomino, Djimsiti; Hateboer, Pasalic, Freuler, Gosens; Malinovskyi, Gomez; Duvan Zapata
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